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1 µM ST034307 inhibits changes in chronotropy in intact mouse right atria with intact SAN but not inotropy in left atria. (A) Dose response curves to show the change in beating rate on cumulative addition of PE to spontaneously beating mouse right atria preparations under control conditions (circles, n = 15) and in the presence of 1 µM ST034307 (squares, n = 12). (B) Dose response curves to show the change in tension generated on cumulative addition of PE to mouse left atria preparations under control conditions (circles, n = 5) and in the presence of 1 µM ST034307 (squares, n = 7). Dose-response curves in (A,B) (solid lines) were fitted using log(agonist) vs. response (three-parameter model) <t>using</t> <t>Graphpad</t> Prism <t>v9.</t> Asterisks indicate significance level for effect of ST034307 compared to control at individual concentrations as determined using 2-way repeated measures ANOVA followed by Šídák’s multiple comparisons test. Data are represented as mean ± SEM; ns = not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
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1 µM ST034307 inhibits changes in chronotropy in intact mouse right atria with intact SAN but not inotropy in left atria. (A) Dose response curves to show the change in beating rate on cumulative addition of PE to spontaneously beating mouse right atria preparations under control conditions (circles, n = 15) and in the presence of 1 µM ST034307 (squares, n = 12). (B) Dose response curves to show the change in tension generated on cumulative addition of PE to mouse left atria preparations under control conditions (circles, n = 5) and in the presence of 1 µM ST034307 (squares, n = 7). Dose-response curves in (A,B) (solid lines) were fitted using log(agonist) vs. response (three-parameter model) <t>using</t> <t>Graphpad</t> Prism <t>v9.</t> Asterisks indicate significance level for effect of ST034307 compared to control at individual concentrations as determined using 2-way repeated measures ANOVA followed by Šídák’s multiple comparisons test. Data are represented as mean ± SEM; ns = not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
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1 µM ST034307 inhibits changes in chronotropy in intact mouse right atria with intact SAN but not inotropy in left atria. (A) Dose response curves to show the change in beating rate on cumulative addition of PE to spontaneously beating mouse right atria preparations under control conditions (circles, n = 15) and in the presence of 1 µM ST034307 (squares, n = 12). (B) Dose response curves to show the change in tension generated on cumulative addition of PE to mouse left atria preparations under control conditions (circles, n = 5) and in the presence of 1 µM ST034307 (squares, n = 7). Dose-response curves in (A,B) (solid lines) were fitted using log(agonist) vs. response (three-parameter model) <t>using</t> <t>Graphpad</t> Prism <t>v9.</t> Asterisks indicate significance level for effect of ST034307 compared to control at individual concentrations as determined using 2-way repeated measures ANOVA followed by Šídák’s multiple comparisons test. Data are represented as mean ± SEM; ns = not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
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1 µM ST034307 inhibits changes in chronotropy in intact mouse right atria with intact SAN but not inotropy in left atria. (A) Dose response curves to show the change in beating rate on cumulative addition of PE to spontaneously beating mouse right atria preparations under control conditions (circles, n = 15) and in the presence of 1 µM ST034307 (squares, n = 12). (B) Dose response curves to show the change in tension generated on cumulative addition of PE to mouse left atria preparations under control conditions (circles, n = 5) and in the presence of 1 µM ST034307 (squares, n = 7). Dose-response curves in (A,B) (solid lines) were fitted using log(agonist) vs. response (three-parameter model) <t>using</t> <t>Graphpad</t> Prism <t>v9.</t> Asterisks indicate significance level for effect of ST034307 compared to control at individual concentrations as determined using 2-way repeated measures ANOVA followed by Šídák’s multiple comparisons test. Data are represented as mean ± SEM; ns = not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
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1 µM ST034307 inhibits changes in chronotropy in intact mouse right atria with intact SAN but not inotropy in left atria. (A) Dose response curves to show the change in beating rate on cumulative addition of PE to spontaneously beating mouse right atria preparations under control conditions (circles, n = 15) and in the presence of 1 µM ST034307 (squares, n = 12). (B) Dose response curves to show the change in tension generated on cumulative addition of PE to mouse left atria preparations under control conditions (circles, n = 5) and in the presence of 1 µM ST034307 (squares, n = 7). Dose-response curves in (A,B) (solid lines) were fitted using log(agonist) vs. response (three-parameter model) <t>using</t> <t>Graphpad</t> Prism <t>v9.</t> Asterisks indicate significance level for effect of ST034307 compared to control at individual concentrations as determined using 2-way repeated measures ANOVA followed by Šídák’s multiple comparisons test. Data are represented as mean ± SEM; ns = not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
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1 µM ST034307 inhibits changes in chronotropy in intact mouse right atria with intact SAN but not inotropy in left atria. (A) Dose response curves to show the change in beating rate on cumulative addition of PE to spontaneously beating mouse right atria preparations under control conditions (circles, n = 15) and in the presence of 1 µM ST034307 (squares, n = 12). (B) Dose response curves to show the change in tension generated on cumulative addition of PE to mouse left atria preparations under control conditions (circles, n = 5) and in the presence of 1 µM ST034307 (squares, n = 7). Dose-response curves in (A,B) (solid lines) were fitted using log(agonist) vs. response (three-parameter model) <t>using</t> <t>Graphpad</t> Prism <t>v9.</t> Asterisks indicate significance level for effect of ST034307 compared to control at individual concentrations as determined using 2-way repeated measures ANOVA followed by Šídák’s multiple comparisons test. Data are represented as mean ± SEM; ns = not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
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1 µM ST034307 inhibits changes in chronotropy in intact mouse right atria with intact SAN but not inotropy in left atria. (A) Dose response curves to show the change in beating rate on cumulative addition of PE to spontaneously beating mouse right atria preparations under control conditions (circles, n = 15) and in the presence of 1 µM ST034307 (squares, n = 12). (B) Dose response curves to show the change in tension generated on cumulative addition of PE to mouse left atria preparations under control conditions (circles, n = 5) and in the presence of 1 µM ST034307 (squares, n = 7). Dose-response curves in (A,B) (solid lines) were fitted using log(agonist) vs. response (three-parameter model) <t>using</t> <t>Graphpad</t> Prism <t>v9.</t> Asterisks indicate significance level for effect of ST034307 compared to control at individual concentrations as determined using 2-way repeated measures ANOVA followed by Šídák’s multiple comparisons test. Data are represented as mean ± SEM; ns = not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
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1 µM ST034307 inhibits changes in chronotropy in intact mouse right atria with intact SAN but not inotropy in left atria. (A) Dose response curves to show the change in beating rate on cumulative addition of PE to spontaneously beating mouse right atria preparations under control conditions (circles, n = 15) and in the presence of 1 µM ST034307 (squares, n = 12). (B) Dose response curves to show the change in tension generated on cumulative addition of PE to mouse left atria preparations under control conditions (circles, n = 5) and in the presence of 1 µM ST034307 (squares, n = 7). Dose-response curves in (A,B) (solid lines) were fitted using log(agonist) vs. response (three-parameter model) <t>using</t> <t>Graphpad</t> Prism <t>v9.</t> Asterisks indicate significance level for effect of ST034307 compared to control at individual concentrations as determined using 2-way repeated measures ANOVA followed by Šídák’s multiple comparisons test. Data are represented as mean ± SEM; ns = not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
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Image Search Results


1 µM ST034307 inhibits changes in chronotropy in intact mouse right atria with intact SAN but not inotropy in left atria. (A) Dose response curves to show the change in beating rate on cumulative addition of PE to spontaneously beating mouse right atria preparations under control conditions (circles, n = 15) and in the presence of 1 µM ST034307 (squares, n = 12). (B) Dose response curves to show the change in tension generated on cumulative addition of PE to mouse left atria preparations under control conditions (circles, n = 5) and in the presence of 1 µM ST034307 (squares, n = 7). Dose-response curves in (A,B) (solid lines) were fitted using log(agonist) vs. response (three-parameter model) using Graphpad Prism v9. Asterisks indicate significance level for effect of ST034307 compared to control at individual concentrations as determined using 2-way repeated measures ANOVA followed by Šídák’s multiple comparisons test. Data are represented as mean ± SEM; ns = not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Journal: Frontiers in Pharmacology

Article Title: Inhibition of adenylyl cyclase 1 by ST034307 inhibits IP 3 -evoked changes in sino-atrial node beat rate

doi: 10.3389/fphar.2022.951897

Figure Lengend Snippet: 1 µM ST034307 inhibits changes in chronotropy in intact mouse right atria with intact SAN but not inotropy in left atria. (A) Dose response curves to show the change in beating rate on cumulative addition of PE to spontaneously beating mouse right atria preparations under control conditions (circles, n = 15) and in the presence of 1 µM ST034307 (squares, n = 12). (B) Dose response curves to show the change in tension generated on cumulative addition of PE to mouse left atria preparations under control conditions (circles, n = 5) and in the presence of 1 µM ST034307 (squares, n = 7). Dose-response curves in (A,B) (solid lines) were fitted using log(agonist) vs. response (three-parameter model) using Graphpad Prism v9. Asterisks indicate significance level for effect of ST034307 compared to control at individual concentrations as determined using 2-way repeated measures ANOVA followed by Šídák’s multiple comparisons test. Data are represented as mean ± SEM; ns = not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Article Snippet: Log[concentration]-response curves, used to estimate EC50s and maximum responses, were calculated using Prism v9 software (GraphPad, CA, United States), by fitting an agonist-response curve with a fixed slope to normalized response data.

Techniques: Control, Generated